A fully equipped laboratory is available for pre-PCR work to be carried out. This laboratory is physically isolated from all PCR and post-PCR areas and stringent anti-contamination protocols are implemented to maintain a DNA-free environment for the preparation of samples for both standard and low-template DNA analysis.
The Nanodrop-1000™ is a full-spectrum UV-vis spectrophotometer that can be used for the quantification of nucleic acids and proteins. It allows for DNA quantification to be performed using minimal sample (approximately 1μl per reading) and has a detection limit in the region of 2ng/μl with an accuracy of ±2ng for [DNA] ≤100ng/μl.
The CFX96™ Real-Time system has six-dye detection capabilities allowing for multiplexing of specific targets is a single assay and can be used for the following applications: Standard Curve (absolute quantitation) Relative Standard Curve Comparative Ct (ΔΔCt) (relative quantitation) Genotyping and Presence⁄ Absence Melt Curve Analysis High Resolution Melting SNP Genotyping Gene Expression Analysis MicroRNA Expression Translocation Analysis Gene Detection Viral Load Analysis
The Veriti® Thermal cycler is a 96-well format modern thermal cycler with full touch screen interface for easy programming and operation. This model also benefits from the VeriFlex ™ PCR block design comprising six independent temperature blocks, which can be programmed individually for rapid optimisation of newly developed methods. This model also allows for new PCR experiments to be designed in both fast and standard run modes allowing more flexibility in cycling conditions and reduction of overall run times.
The 3130 Genetic Analyser is a 4-capillary electrophoresis system that can be used to run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening as well as de novo sequencing and re-sequencing. This model benefits from the addition of an automated polymer pump which reduced the time required for machine maintenance and polymer waste.
Illumina Next Generation Sequencing Platform
Managed by NU-OMICS
“NU-OMICS aims to provide a cost effective, academia-led, DNA sequencing service to both academia and industry alike. Established within the multi-omics capabilities at Northumbria University, we offer competitive pricing for single or multiple projects where we can offer quotations for grant submissions and aid in the design of experiments to optimise your sequencing output.”
Dr Darren Smith
This technique is used for the separation and comparison of dye and ink components from trace evidence material.
Used for the chemical elucidation and comparison of organic trace evidence material (e.g. Fibres, Paints, Inks etc.)
This system allows the determination and comparison of the refractive index of microscopic glass fragments encountered in forensic casework.
Used for the elemental analysis and comparison of inorganic trace evidence material (e.g. glass).
Separates and identifies mixtures of organic compounds (e.g. polymers).
A database of magnetic resonance imaging (MRI) scans of the head and neck of 60 volunteers is available to NUCFS graduate students interested in craniofacial morphology.
The database was collected by Prof. Evison and Prof. Iain Wilkinson of Sheffield University with the support of the Royal Society for research into craniofacial identification.
The database has been used to investigate relationships between soft and hard tissue measurements (see Figure) in research conducted by Daniel Fu Ren (Liaoning Medical School, China) and Kelly Kunyang Fang (Yale University) working in Prof. Evison’s laboratory.
The Magna Database is a database of 3000 3D facial images collected at the Magna Science Adventure Centre as part of a research project in forensic facial comparison sponsored by the FBI.
The database is available to NUCFS postgraduate research students interested in craniofacial morphology and shape variation.
The database was collected using a 3D stereophotographic biometric camera, which captured at 2D images that can be used to collect precise 3D measurements and generate 3D face surface. Some of the volunteers in the Magna Database also volunteered for the MRI head and neck database, meaning that comprehensive 3D surface and volume data is available for to potential students interested in craniofacial identification.